Bright, Inexpensive Ly-6g Antibody

February 16, 2016

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

APC-Cyanine7 Anti-Mouse Ly-6G

Catalog Number:

25-1276

Browse Similar Products: Ly-6g Flow Cytometry Antibodies

Peritoneal leukocyte populations were being identified following heat-killed E. coli injection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total Peritoneal Leukocytes

Primary Incubation

1:400 Ly6g-APC/Cy7 - 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II (40mW Red Laser)

Results Summary

Tonbo's Ly-6g antibody (Clone 1A8) allowed for clear identification of neutrophils when combined with CD11b. Neutrophil influx was noted at 4 hours following heat killed E. coli IP injection.

Additional Notes

Gated on CD45+7AAD-Singlets

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Summary

The Good

Price and staining results

The Bad

None

The Bottom Line

Clear identification of population of interest at a low cost.

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