LDHA Antibody from Cell Signaling

February 17, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

LDHA Antibody

Catalog Number:

3582S

This antibody was used to detect LDHA expression in human cancer cell lines in response to cetuximab treatment.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cancer Cells whole lysates

Primary Incubation

1:2000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:5000, 1 hour, RT

Tertiary Incubation

None

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody detects LDHA expression by western blot.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Signal is strong and specific.

The Bad

None

The Bottom Line

This is a perfect antibody for detection of LDHA by western blot.

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