Biorbyt
Yeats2 antibody
orb215220
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Browse Similar Products: YEATS2 Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk in PBST
1:3000 anti-rabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Antibody only picks up strong nonspecific bands, making it useless. It's possible that the weak band is specific, though it is hard to tell, especially because of the stronger nonspecific band above it. We confirmed candidate 13 as a true knockout using sequencing subsequently.
None
Relatively cheap antibody
Strong non-specific band
Wouldn't recommend.