Boster
Anti-UCP1 antibody
PA1982
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We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis.
Western Blot
Subcutaneous fat and brown fat tissue
1:2000, overnight
3% BSA
5% fat-free milk in PBST
1:10000
Although there are some non-specific bands, we still can get the strong and clear bands that we want to.
None
The WB band is strong even when you use high dilution concentration.
Every time we need to thaw it because it was stored in-80 C.
Overall this antibody is good to check UCP1 levels using WB technology.