Cell Signaling Technology
Phospho-Cofilin (Ser3) Antibody
3311S
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Aortic smooth muscle cell migration into tunica intima is a major cause of restenosis. We have tested various signaling molecules levels in vascular smooth muscle cells to understand the mechanism of their migration. Here, we tested the time-course effect of a chemokine on phosphorylation of cofilin in human aortic smooth muscle cells.
Western Blot
Aortic Smooth Muscle Cell lysates
5% skim milk, 1 hr at room temperature
5% BSA
1:1000 dilution/ over night / 4 deg C in TBST + 5% BSA
Goat anti-rabbit, 1:4000, room temperature for 1 hr in TBST + 5% skim milk
Horseradish peroxidase (HRP)
Time-course treatment of chemokine has no significant effect on cofilin phosphorylation.
None
Very strong signal with recommended dilutions & very specific
Needs optimization.
Very good antibody with strong and specific signal.