Chemokine Response on ARP2 Expression in Smooth Muscle Cells

Physiology
University of Tennessee Health Science Center

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Company:

Cell Signaling Technology

Product Name:

ARP2 Antibody

Catalog Number:

3128S

Aortic smooth muscle cell migration into tunica intima is a major cause of restenosis. We have tested various signaling molecules levels in vascular smooth muscle cells to understand the mechanism of their migration. We tested the time-course effect of a chemokine on expression of ARP2 in Human aortic smooth muscle cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Aortic Smooth Muscle Cell lysates

Primary Incubation

5% skim milk, 1 hr at room temperature

Blocking Agent

5% skim milk

Secondary Incubation

1:1000 dilution/ overnight / 4 deg C

Tertiary Incubation

Goat anti-rabbit, 1:5000, room temperature for 1 hr

Detection

Horseradish peroxidase (HRP)

Results Summary

Time-course effect of chemokine has no effect on PKC alpha expression

Additional Notes

None

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Summary

The Good

Very specific and no background

The Bad

Only for WB not good for other applications like IP/IF/IHC

The Bottom Line

Very good antibody for just western blot applications

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