Coupling Antigens to Beads

Kansas State University
Veterinary Diagnostic Laboratory
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

Luminex

Product Name:

Antibody coupling kit

Catalog Number:

40-50016

The aim of this experiment was to couple several different antigens to magpix beads. These coupled beads were further used with equine serum to identify different antibodies in the animal.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Coupled beads + serum

Protocol Overview

The kit was used to couple magpix beads to different viral antigens. For example, west nile virus antigen. These beads are magnetic beads that after coupling were used on the magpix.

Tips

Make sure you make the EDC right before adding it.

Results Summary

The kit performed very well and allowed for optimal coupling of the antigens to the beads.

Additional Notes

None

Related Categories

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Summary

The Good

Very easy to use.

The Bad

None

The Bottom Line

Great coupling kit for antibodies, antigens, oligonucelotides. Etc.

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