Good Antibody to Detect FGF-2 in Mouse Tissues

Department of Zoology
Ranchi University, Ranchi
Research Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology Inc

Product Name:

FGF-2 antibody (147)

Catalog Number:

Sc-79

The present study was conducted to test the role of specific treatment on FGF-2 expression in mouse retina. Protein was extracted from non- treated or treated mouse retina, equal amount of proteins were resolved in 10% SDS-PAGE, and immunoblotted with specific FGF-2 antibody from Santa Cruz Biotechnology.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse retinal extract

Primary Incubation

Dilution (1:500) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius

Blocking Agent

5% skimmed milk prepared in TBST

Secondary Incubation

Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT

Tertiary Incubation

None

Detection

Enhanced chemiluminiscent detection in X-Ray film

Results Summary

FGF-2 antibody from Santa Cruz Biotech is an excellent antibody which has given a specific band at the desired molecular weight. A positive effect of FGF-2 expression was observed in response to treatment.

Additional Notes

None

Related Categories

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Summary

The Good

Gives very specific band

The Bad

NA

The Bottom Line

Excellent antibody

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