Chemokine Response on PKC aplha Expression in Smooth Muscle Cells

Physiology
University of Tennessee Health Science Center

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Company:

Santa Cruz Biotechnology

Product Name:

PKC alpha antibody (C-20)

Catalog Number:

sc-208

We tested the time-course effect of a chemokine on expression of PKC alpha in Human aortic smooth muscle cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Aortic Smooth Muscle Cell lysates

Primary Incubation

5% skim milk, 1 hr at room temperature

Blocking Agent

5% skim milk

Secondary Incubation

1:500 dilution/ overnight / 4 deg C

Tertiary Incubation

Goat anti-rabbit, 1:5000, room temperature for 1 hr

Detection

Horseradish peroxidase (HRP)

Results Summary

Time-course effect of chemokine has no effect on PKC alpha expression.

Additional Notes

None

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Summary

The Good

The antibody is very good and it gives a single non-specific band.

The Bad

Need high concentration

The Bottom Line

The antibody is very specific in detecting PKC alpha in aortic smooth muscle cells.

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