Isotype Control for K9 Immunophenotyping

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

BioLegend

Product Name:

PE Mouse IgG1, κ Isotype Ctrl (FC)

Catalog Number:

400114

The purpse of the experiment was detection of B cell/T cell lymphoma using flow cytometry. We used this isotope control as a means to gate out the negative population and distinguish between stained and unstained cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood in EDTA

Primary Incubation

1ul of the isotope for 30mins in the dark at 4 degrees.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Serves as a good isotype control.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Serves the purpose of the experiment.

The Bad

None

The Bottom Line

Great isotype control.

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