Cell Signaling Technology
Akt
9272S
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Browse Similar Products: Akt Western blot Antibodies
Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We were testing this antibody to analyze Akt in cancer cells through western blotting.
Western Blot
Cancer cells
1:1000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
30 ugs of protein samples were used for this western blot to detect Akt in PC3 cell lysates. I observed a very strong band using anti-Akt antibody around 60 KD molecular weight.
None
Very good antibody for western blot
Good antibody for western blot