Western Blot Analysis of Akt

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt

Catalog Number:

9272S

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We were testing this antibody to analyze Akt in cancer cells through western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:1000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

30 ugs of protein samples were used for this western blot to detect Akt in PC3 cell lysates. I observed a very strong band using anti-Akt antibody around 60 KD molecular weight.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good antibody for western blot

The Bad

None

The Bottom Line

Good antibody for western blot

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