Good Antibody for Phoshp-SRC

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-Src Family (Tyr416)

Catalog Number:

2101

I am investigating the role of the SRC-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total SRC antibody from Cell Signaling and it is showing very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

RT, 1h, 5% milk in TBST

Secondary Incubation

RT, 1h, 5%mil in TBST, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad clarity

Results Summary

Very good antibody for the detection of phospho SRC.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Shows unspecific bands sometimes

The Bottom Line

Good antibody for phospho-SRC

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