Very Good Phospho-PDK1 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-PDK1 (Ser241) Antibody

Catalog Number:

3061

I am investigating the role of the PDK1-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total PDK1 antibody from Cell Signaling and they are showing very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

4°C, 5%BSA, 1:1000, overnight

Blocking Agent

RT, 5% milk-TBST, 1h

Secondary Incubation

RT, 5% milk-TBST, 1h

Tertiary Incubation

NA

Detection

Bio-Rad Clarity

Results Summary

Shows good bands with no non-specific binding

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Perfect antibody for phospho-PDK1 detection

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