Mouse Skin Wound CD4 Immunofluorescence Staining

January 14, 2016

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified rat anti mouse CD4

Catalog Number:

550280

The aim of this study was to detect CD4 cells in mouse skin wounds. This antibody works well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin wounds

Primary Incubation

0.3125ug/ml, 45 min RT

Blocking Agent

10% goat serum

Secondary Incubation

Alexa flour 488 goat anti rat

Tertiary Incubation

NA

Detection

Alexa flour 488

Results Summary

This antibody strongly detected CD4+ lympnocytes in mouse skin wounds.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use, less expensive, good signal

The Bad

None

The Bottom Line

Recommended

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