Cell Signaling Technology
GLI1 (L42B10) Mouse mAb
2643S
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The goal of this experiment was to test the induction of Gli1 protein in NIH3T3 cells after Smo agonist (SAG) treatment
Western Blot
NIH3T3 cells
1:1000 dilution in blocking buffer for 16 hr at 4 deg
5% Milk powder in 1x TBS-T (0.1% Tween)
Donkey-anti mouse IgG HRP secondary antibody for 1 hr at room temperature
NA
ECL pico for 5 min on a film
NIH3T3 cells nicely respond to Smoothened agonist (SAG) treatment and induce Gli1 induction. The antibody detects a specific band at ~150 kDa. May be due to low levels of abundance, one needs to use ECL pico or sometimes ECL femto, to detect a good signal.
None
Specific signal at ~150 kDa
Tricky sometimes due to low abundance
When ECL pico or ECL Femto are used for detection, it works very well