BD Bioscience
Purified Mouse Anti-PKA[C]
610980
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Browse Similar Products: PKA Western blot Antibodies
One of the goals of my project is to carry out protein-protein interactions with components that are relevant to the hedgehog signaling. A first step in the process is to test the antibodies to determine the expression in either over-expression or endogenous levels. The goal of this experiment was to test the expression level of PKA catalytic subunit in NIH-3T3 cells.
Western Blot
NIH-3T3 whole cell lysate_50 µg
1:500 dilution in blocking buffer and incubation for 16 hr at 4 deg on a rotator
5% Milk in 1x TBS-T (0.1%)
1:5000 dilution in blocking buffer and incubation for 1 hr at 25 deg on a rotator
None
ECL
This is a fantastic antibody that gives a specific signal at 40 kDa and no non-specific bands. I had used it a couple of times and never it had never failed in my hands.
Specific signal and no background issues
Excellent antibody for Western Blotting