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APC anti-mouse CD103 Antibody
350216
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Splenocytes were analyzed for the presence of CD103+ DC under various conditions (eg tumor bearing vs non tumor bearing mice)
Flow Cytometry
Mouse splenocytes
1:100, 15min, 4C
Blocking Buffer containing sera (rat, rabbit, human, goat)
NA
Flow cytometry (BD) LSRFortessa
Good staining of CD103 amongst splenic DC (identified by CD11c and MHCII)
None
Easy to identify if DCs are plenty
Difficult to identify CD103+ DC if DC are sparse (eg homeostasis)
Good antibody to identify CD103+DC