Easy to Use DNase Kit

rush university medical center
orthopadic
research scientist

Overall

Quality of Results

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Company:

Qiagen

Product Name:

RNase-free DNase set

Catalog Number:

79254

This small kit comes with a DNase enzyme tube and two buffer tubes. It is easy to make working solution and only need 15 min at room temperature for digestion. Good and reliable results.

Experimental Design and Results Summary

Application

DNA digestion during RNA extraction

Starting Material

Cell lysates

Protocol Overview

For the Qiagen RNA extraction kit, this DNase digestion is an optional step depend on the sample. By selecting this step, just mix 70ul of RDD buffer with 10ul of DNase enzyme and add onto the column. Leave the column at room temperature for 15 min and it is ready to go to next step.

Tips

Works at room temperature

Results Summary

This kit can digest DNA very well and leaves out a clean RNA after.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to mix and use

The Bad

Not found yet

The Bottom Line

The Qiagen RNase-free DNase kit is a very useful and easy to use kit. It works well in RNA extraction processes and results are reliable.

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