Cell Signaling Technology
α/β-Tubulin Antibody
2148
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The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.
Immunofluorescence
Caco-2 cells
1:50 diluted in 1xPBS, incubation for 1h at room temperature
No blocking
Cy3-coupled secondary antibody, 30 min incubation at room temperature
No
Fluorescence microscopy
We were able to detect the microtubules within the cells by using this antibody.
None
Specific antibody
Low dilution factor
Good antibody for the detection of microtubules with immunofluorescence.