eBioscience
Anti-Human CD3 PerCP-Cyanine5.5
45-0037
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The aim of this research project was to immune phenotype lymphocyte subsets in human peripheral blood using minimal amount of samples.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and then resuspended in 300ul of FACS buffer and taken to flow cytometry.
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Flow cytometry
This antibody worked very well to separate CD3+ cells in human peripheral blood.
In the image you see lymphocytes that are stained with the described CD3 antibody and additionally with a CD4 antibody
Very good separation of CD3+ cells
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This is an excellent antibody to identify CD3+ cells in human peripheral blood.