Silver Staining Kit

December 11, 2015

Harvard
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

SilverQuest™ Silver Staining Kit

Catalog Number:

LC6070

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Application

Silver stain proteins and DNA in polyacrylamide gels

Starting Material

Polyacrylamide gels

Protocol Overview

PAGE gels were briefly washed with deionized water and fixed in fixative. After fixative gels were treated with individual kit reagents for designated time. Completed staining procedure is completed in about 2 hours.

Tips

Always use deionized water

Results Summary

Excellent protein gel silver staining was achieved each time.

Additional Notes

None

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Summary

The Good

Easy to use and fast silver staining kit

The Bad

None

The Bottom Line

Kit is very easy to use and always gives reliable results. There is fast staining protocol also available that gives results in 30 minutes. Kit also comes with destaining solutions that makes it ideal for Mass-Spec analysis.

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