Sirt6 Expression in Neuronal Cells

Project JRF
Centre for Cellular & Molecular Biology
Postdoctoral Researcher

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Company:

Abcam

Product Name:

Anti-SIRT6 antibody

Catalog Number:

ab62739

This product was used to check Sirt6 expression levels in neuronal cells in different experimental conditions. The product is standardised for western blot and works well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Neuronal cell (N2A) cell lysate.

Primary Incubation

1:1000(1ug/mL) in 5%BSA-1XTbst, 4c, overnight

Blocking Agent

5%BSA-1XTbst, 1h, room temperature

Secondary Incubation

Anti rabbit, 1:5000 in 5%BSA-1XTbst, room temperature, 2h

Tertiary Incubation

NA

Detection

Luminol-chem doc instrument

Results Summary

Sirt6 expression is changing in neuronal (N2a cells).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Gives a prominent band

The Bad

Nothing bad

The Bottom Line

Recommended to use for western blot.

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