Phospho-Akt (Ser473) Antibody from Cell Signaling

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) Antibody

Catalog Number:

4060

This antibody was used to detected changes of Akt phosphorylation in response to cetuximab treatment in a time dependent manner.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detects changes of phosphorylation of Akt at S473 in response to cetuximab treatment without any non-specific bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects a specific band and the signal is strong

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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