Labeling of CD4 Positive Cells in Human PBMC Samples

November 18, 2015

Anaesthesiology
LMU Munich
Researcher

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Company:

BioLegend

Product Name:

FITC anti-human CD4 Antibody

Catalog Number:

317407

Info: View Product View Product Specs

Browse Similar Products: CD4 Antibodies

This antibody gives quick staining, reliable results, great signal, and good value.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 mins on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

As shown, good discrimination between CD4 negative and -positive cells in human PBMCs was obtained after Ficoll gradient centrifugation, compared to isotype control ab.

Additional Notes

Wash three times after incubation

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Summary

The Good

Quick, good value, affordable

The Bad

None

The Bottom Line

Quick screening for CD4 expression on a variety of cells.

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