Transfection Using Fugene HD

UPENN
Ophthalmology
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Promega

Product Name:

Fugene HD

Catalog Number:

E2311

This product was used for transfection of JEG3 cell lines.

Experimental Design and Results Summary

Application

Transfection

Starting Material

JEG3 cells

Protocol Overview

This reagent works well even for tough cell lines like JEG3.

Tips

Ratio of 3:1

Results Summary

We used a ratio of 3:1, 200ng plasmid to 0.6 ul of Fugene HD in 96-well plate.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

I have used even 3 months after expiration and it's fine.

The Bad

It may not work for all cells. You should make sure to try different ratios or number of cells before the pilot experiment.

The Bottom Line

It's easy to work in medium containing serum and even antibiotics.

Join the discussion