Nod1 Expression in Mouse T Cells

November 04, 2015

Immunology
Northwestern University
Grad Student

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Company:

Cell Signaling Technology

Product Name:

Nod1 Antibody

Catalog Number:

3545

Info: View Product View Product Specs

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The aim of the research was to determine the levels of Nod1 in differentiated mouse Th1 and Th17 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:4000, overnight, rocking, 4 degrees

Blocking Agent

10% Milk

Secondary Incubation

1:10,000 in 5% milk, 30 min

Tertiary Incubation

NA

Detection

Chemiluminescence

Results Summary

Low background and sharp bands

Additional Notes

None

Related Categories

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Summary

The Good

Easy to optimize

The Bad

Nothing negative to comment

The Bottom Line

Good antibody for the detection of Nod1 by Western blot

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