Anti-GFP to Study Protein Co-Localization from Cytoplasm to Nucleus

November 03, 2015

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Anti-GFP, N-terminal antibody produced in rabbit.

Catalog Number:

G1544

Browse Similar Products: GFP Western blot Antibodies

This product was helpful in an experiment to visualize the localization of my protein of interest from cytoplasm to nucleus.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa cell lysate

Primary Incubation

2hr at room temperature

Blocking Agent

Blotto from SC

Secondary Incubation

1hr at room temperature

Tertiary Incubation

NA

Detection

Amersham ECL

Results Summary

Easily produced publication quality blots.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very easy to optimize

The Bad

Nothing noticed.

The Bottom Line

Highly recommended

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