Sigma-Aldrich
4′,6-Diamidino-2-phenylindole dihydrochloride
D8417
I often use immunofluorescence assays to determine protein localization in the cell. Generally, my protein of interest resides in the cytoplasm of the cell and I use DAPI for nuclear staining. It works amazingly well for every single experiment. DAPI from sigma does not give any non-specific staining and works immediately, in seconds.
Immunoflurescence
Cervical cancer cells
Cells were fixed in paraformaldehyde, stained with anti-AKAP4 antibody, and followed by secondary antibody. 10 microlitres of DAPI solution (1mg/ml) was added at the end to stain the nuclei of the cells. Images were captured using a fluorescece microscope.
For DAPI staining, less is more.
It gives very specific and sharp blue color in the nuclei of the cells.
None
Highly specific, sharp staining
Perfect product for nuclear staining of the cells