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Pacific Blue™ anti-mouse CD3 Antibody
100214
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I used this to identify the CD3+ T Cell population in the mouse spleen to use for downstream research.
Flow Cytometry
Mouse spleen
1X10^6 suspension cells were incubated with 0.5ul PB anti-mouse CD3 in 50ul FACS buffer for 30 min at 4oC.
0.5ul CD16/CD32 for 10 min at 4oC
NA
The graph shows it is a very good Ab to gate CD3 population.
None
Easy to use.
I recommend this Ab to other colleagues.