Qiagen (SABiosciences)
ChampionChIP Human RNAP II Assay Kit
GA-111
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This kit is useful to confirm your ChIP assay is working. It comes with RNAP II antibody along with positive and negative control primers. The kit provides the necessary ChIP grade antibody and primers to determine if ChIP lysates are valid. I enjoy how the kits comes with all major components sans ChIP lysate to perform the verification ChIP.
ChIP
NA
IP ChIP lysates with RNAP II antibody. After processing IP samples to obtain DNA, run PCR with negative and positive primers to assay.
Follow protocol
Using this kit with my ChIP protocol, I showed enrichment with the positive control primers vs antibody control and no enrichment with negative control primers.
Comes with antibody and primers needed
None
Comes with what you need
This is a nice kit to verity ChIP protocols.
Product Name: RNA Polymerase II ChIP-GradeSupplier Page