Good Secondary Antibody

October 21, 2015

Medicine
HMS
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Goat Anti-Mouse IgG (H + L)-HRP Conjugate

Catalog Number:

1706516

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I used this antibody for studying protein-protein interaction between ESAT6 and novel peptides identified using dot-blot western technique.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Purified protein

Primary Incubation

1:2000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X PBS

Secondary Incubation

1:5000 at room temperature for 2 hours

Tertiary Incubation

None

Detection

Anti-mouse HRP, TMB

Results Summary

ESAT6 and de novo peptide HCL2 interaction was detected by overlaying ESAT6-FLAG tag protein and detecting the interaction by anti-FLAG antibody.

Additional Notes

None

Image Gallery

Summary

The Good

Good secondary antibody, gives nice signal

The Bad

None

The Bottom Line

Specific anti-mouse secondary antibody, gives minimal background

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