Good Anti-FLAG Tag Antibody

October 27, 2015

Medicine
HMS
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Catalog Number:

F3165-.2MG

Browse Similar Products: FLAG Antibodies

I used this antibody for studying protein-protein interaction between ESAT6 and novel peptides identified using dot-blot western technique.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Purified protein

Primary Incubation

1:2000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X PBS

Secondary Incubation

1:5000 at room temperature for 2 hour

Tertiary Incubation

None

Detection

Anti-mouse HRP, TMB

Results Summary

ESAT6 and de novo peptide HCL2 interaction was detected by overlaying ESAT6-FLAG tag protein and detecting the interaction by anti-FLAG antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Antibody is specific and gives neat results

The Bad

None

The Bottom Line

Highly specific, no cross reactivity

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