DNeasy Blood Tissue Kit

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

DNeasy Blood Tissue Kit

Catalog Number:

69504

We are assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated by density centrifugation from whole bone marrow suspensions and grown in the presence of GM-CSF for 5-7 days. We then use flow cytometry, RT-PCR, western blots etc. to assess changes in dendritic cells after AhR activation.

Experimental Design and Results Summary

Application

DNA isolation from murine tissue

Starting Material

Lung Tissue

Protocol Overview

We started with lung tissue, and followed the protocol to separate DNA.

Tips

Perform Optional Steps

Results Summary

We get clean results, and good yield with this kit every time we use it.

Additional Notes

None

Image Gallery

Summary

The Good

Everything comes in the kit.

The Bad

None

The Bottom Line

I would recommend this product if you are wanting to extract DNA from whole tissue samples.

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