Thermo Fisher Scientific (Invitrogen)
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate
A-11037
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I do a lot of immunofluorescent staining in the lab, often using rabbit-derived primary antibodies to stain my antigen of interest. To visualize the rabbit anti-mouse stain, I use this product.
Immunofluorescence
Pre-fixed frozen mouse small intestine tissue section
1:100 in 1% BSA in PBS overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rabbit AlexaFluor594 was used at 1:200 in PBS and was incubated on the section for 1 hour at room temperature in the dark
None
Zeiss LSM 700 confocal
The rabbit antibodies are clearly visualized without high background or additional artifacts.
Easy to use and very clear staining
None to mention
The product is great for visualizing rabbit primary antibodies in the AlexaFluor 594 channel.