Good APC Conjugate to Stain CSF-1R Myeloid Cells in Mouse

October 09, 2015

Rheumatology
University of California
Graduate Student

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Company:

BioLegend

Product Name:

Anti-mouse CSF1R

Catalog Number:

135509

Info: View Product View Product Specs

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In vitro cultured bone marrow derived cells were checked for the expression of CSF-1R under osteoclast differentiation conditions in mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow cells in vitro cultures

Primary Incubation

1:200 dilution of CD11b and anti-CSF1R

Blocking Agent

Fc block in FBS/PBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Excellent staining on even a small population of cells that were positive for both CD11b and CSF1R

Additional Notes

None

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Summary

The Good

Good signal due to APC and specificity

The Bad

Little bit expensive compared to others

The Bottom Line

Overall good, specific antibody to produce excellent signal for detection by flow cytometry.

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