Western Blot Analysis of Phospho-EGFR (Y1173)

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

Phospho EGFR

Catalog Number:

44794G

Info: View Product View Product Specs

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Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. This product was used to test the phosphorylation of EGFR after treatment with EGF.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:3000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

Very good antibody to measure the activation of EGFR using this antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good antibody for western blot and ICC as well.

The Bad

None

The Bottom Line

Best antibody for both western blot and ICC

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