Western Blot Analysis of EGFR

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

EGFR antibody

Catalog Number:

SC-03

Info: View Product View Product Specs

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Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. This product was used to detect EGFR through western blot analysis in cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:3000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

Antibody detected EGFR at molecular weight approximately 180kd.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good antibody for western blot and ICC as well.

The Bad

None

The Bottom Line

Very easy to detect EGFR in western blot analysis

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