K9 Immunophenotyping Using CD14 BV650 for Cell Surface Staining

September 30, 2015

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-human CD14

Catalog Number:

301835

Info: View Product View Product Specs

Browse Similar Products: CD14 Antibodies

This product was used to perform immunophenotyping on dog samples for diagnosis of lymphoma. BV650 CD14 was selected to stain for monocytic and a few granulocytic populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood

Primary Incubation

5ul of the antibody for 20 mins in the dark at RT.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD fortessa

Results Summary

Cells showed staining with the marker and I was able to distinguish between the monocytes and the macrophages.

Additional Notes

None

Related Categories

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Summary

The Good

Good staining obtained.

The Bad

Have not noticed any yet.

The Bottom Line

Good marker.

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