Western Blot Analysis of MFN1

Biology
University of Houston
Graduate student

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Quality of Results

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Company:

Novus Biologicals

Product Name:

MFN1 antibody

Catalog Number:

NBP1-71775

Info: View Product View Product Specs

Browse Similar Products: MFN1 Antibodies

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. In this experiment, we were studying pro-fusion proteins of mitochondria in cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:1000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

We were able to detect MFN1 with this antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Works well for Western blot

The Bad

None

The Bottom Line

Good antibody for western blotting.

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