Anti-Human CD4 Antibody for FACS Analysis

October 08, 2015

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Human CD4 APC

Catalog Number:

17-0049

Info: View Product View Product Specs

Browse Similar Products: CD4 Antibodies

I use this antibody for the staining of human CD4+ T cells in PBMCs and subsequent FACS analysis. This antibody was an affordable price so I selected this one.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:500 in 100uL FACS Buffer 4 degrees for 1h

Blocking Agent

0.5% BSA FACS buffer

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Becton Coulter CyAn

Results Summary

I used to get distinct CD4+ population among PBMCs after gating on lymphocytes.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of optimization and quality of results

The Bad

Weak staining

The Bottom Line

Moderate quality results with cheaper price

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