eBioscience
Anti-Mouse/Rat IL-17A PE
12-7177-81
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Here, we wanted to examine the percentage of IL-17A+ T-cells in healthy vs diseased mice. To do this, we utilized this antibody to stain T-cells producing IL-17A following ex vivo stimulation.
Flow Cytometry
PMA/ionomycin-stimulated single cell suspension from mouse small intestine
1:100 in staining buffer for 25 minutes at 4 degrees
FcR Block
NA
BD LSR II flow cytometer
IL-17A-producing T-cells isolated from mouse small intestine and stimulated ex vivo stain clearly with anti-IL-17A.
Cells were fixed and permeabilized using the eBioscience Foxp3 staining kit.
The antibody stains IL-17A-producing T-cells very clearly
None to mention
The antibody is very good for staining IL-17A-producing T-cells.