Simple to Use Non-Hazardous G-250 Coomassie Stain

September 24, 2015

Grambling State University
Biological Sciences
Assistant Professor

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Company:

Life Technologies

Product Name:

SimplyBlue Safestain

Catalog Number:

LC6060

After electrophoretic separation of protein samples, it is often useful to stain the gel for proteins. While many companies offer non-hazardous G-250 formulations, this product was selected mainly because we order many other things from Life Technologies. It also works well and Life Technologies provides excellent documentation.

Experimental Design and Results Summary

Application

Protein staining

Starting Material

Rat liver tissues and rat hepatoma cell lines

Protocol Overview

After electrophoresis, the gel is washed in water to wash out interfering SDS. The gel is stained for 1 hour at room temperature on a shaker then destained with water for at least 1 hour at room temperature.

Tips

Definitely read the manual for many good tips

Results Summary

15 ug of total lysates were separated on 4-16% SDS-PAGE and stained for 1 hour followed by destaining.

Additional Notes

None

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Summary

The Good

Simple and safe just like the name

The Bad

More expensive than competitors

The Bottom Line

Maybe it's just in my head but this seems to work a little better than the Bio-Rad Bio-Safe stain. The manual is very informative. I suppose you could just download that PDF and use the information with a different manufacturer's stain.

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