PALL Life Sciences
BioTrace PVDF
66543
This product is used in western blot applications. Specifically, proteins are transferred from polyacrylamide gels to this PVDF and then probed with antibodies.
Western Blot
None
Cut out a piece of PVDF that fits your gel from the roll. Rinse it in methanol to "activate" it and then place on top of the protein gel. Transfer proteins onto the gel (I do 300 mA for 90 minutes) and then rinse PVDF with wash buffer. Block and then probe PVDF with antibodies to detect protein of interest.
Be careful in cutting the PVDF
Proteins attach well to this membrane and it is suitable for western blotting for phospho and non-phospho proteins.
Easy to use
Good source of PVDF membrane for western blot