Gilson
Microman M100
F148504
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There are multiple techniques for the sampling of murine vaginal mucosa, including PVA swabs and PBS washing. For minimal invasiveness and speed, PBS washing is often employed depending on local animal facility guidelines. This positive displacement pipette, when used with its corresponding tips, provide a convenient and minimally invasive method for vaginal PBS washing.
In vivo mucosal sampling
None
Using the pipette's corresponding tips, 33 ul of PBS is used to wash the murine vaginal vault three times, obtaining a final volume of ~100 ul. The mucosal wash is kept on ice and centrifuged at 10000 rpm for 10 min at 0C to pellet cellular debris before the supernatent is stored at -20C until required (for ELISAs for example). Mice need to be remained anaesthetised during the period of the procedure (approx 2 mins).
Change PBS source between murine groups, and change tips between individual mice.
Consistent volumes of around 100 ul are obtained following the three 33 ul PBS washes. If samples are treated properly and kept on ice and subsequently frozen, total IgG and total IgA concentrations are consistent between mice and murine strains using this sampling method. The speed and ease of use, along with the minimal distress and physical invasiveness make this an attractive mucosal sampling procedure.
Can hold volumes from 10 to 100 ul
Functionality, design and speed of delivery
An essential pipette for the minimally invasive procedure of sampling murine vaginal mucosa by PBS washing.
Product Name: MICROMAN® Positive-Displacement Pipette - M100, 10 - 100µLSupplier Page