Excellent Antibody to Detect Pak2 in Mammalian Cell Line

Physiology
University of Tennessee HSC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Pak2 Antibody

Catalog Number:

2608

Info: View Product View Product Specs

Browse Similar Products: PAK2 Antibodies

Pak2 levels were detected in mammalian cells treated with growth factor. Equal proteins were resolved, western transferred and probed for Pak2. Antibody selection was based on information provided in Cell Signaling's Pka2 Ab flyer.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysate

Primary Incubation

1:1000 (5%skimmed milk in TBST), Overnight at 4 degrees Celsius

Blocking Agent

5% Skimmed milk in TBST

Secondary Incubation

1:4000 (5%skimmed milk in TBST), 1h at RT

Tertiary Incubation

NA

Detection

ECL

Results Summary

Got highly specific band of Pak2

Additional Notes

None

Related Categories

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Summary

The Good

Highly specific

The Bad

None

The Bottom Line

Excellent antibody

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