Cell Signaling Technology
Phospho-eIF4E (Ser209) Antibody
9741
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Phospho-eIF4E (Ser209) levels were detected in mammalian cells treated with PDGF for various time points. Selection of antibody was based on information provided in Cell Signaling Technology's antibody flyer.
Western Blot
Whole cell lysate
1:1000 (5%BSA in TBST), Overnight at 4 degree Celsius
5% Skimmed milk in TBST
1:4000 (5%skimmed milk in TBST), 2h at RT
NA
ECL
Got a highly specific band at around 25 kDa. Growth factor showed time dependent increase of eIF4E phosphorylation.
None
Highly specific for peIF4E
This is an excellent antibody. I always recommended to everyone.