Product to Study Whether Protein of Interest is Localized in Cytoplasm or Nucleus

September 14, 2015

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma-Aldrich

Product Name:

Anti Rabbit IgG-FITC

Catalog Number:

F9887

Browse Similar Products: Secondary IgG Antibodies

We are studying our protein of interest's mechanism of action because ideally it is in the cytoplasm but based off of literature we predicted that it was going to be in the nucleus.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

IMR cell line

Primary Incubation

1:300

Blocking Agent

2% BSA

Secondary Incubation

1:500

Tertiary Incubation

NA

Detection

By Olympus confocal

Results Summary

My protein of interest is under mutated condition thus localized in nucleus.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It is a very good secondary anti rabbit IgG FITC

The Bad

None that I have found

The Bottom Line

Highly recommend to use.

Share your experience with other scientists. Write a Review! »

Join the discussion