Santa Cruz Biotechnology
ERK 2 (C-14) Antibody
sc-154
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Erk2 antibody from Santa Cruz Biotechnology was used to test the time course effect of PDGF on Erk2 levels in mammalian cells. Equal amounts of proteins were resolved in 10% SDS-PAGE, western transferred on a nitrocellulose membrane and immunoblotted with Erk2 antibody.
Western Blot
Whole cell protein lysate
1:1000 (5%skimmed milk in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST
1:4000 (5%skimmed milk in TBST), 1h at RT
NA
ECL
Erk2 antibody showed excellent bright signal with no background noise.
None
Got excellent signal
Overall, Erk2 from Santa Cruz Biotech is an excellent antibody.