CD23 PE Flow Cytometric Stain

August 21, 2015

Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD23

Catalog Number:

553139

BD CD23 PE flow cytometric stain works great at 1:200 final concentration. Allows great discrimination between transitional and mature B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This CD23 PE flow stain works great at 1:200 final concentration and requires minimal compensation with other colors.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

CD23 PE stain works great

The Bad

None

The Bottom Line

CD23 PE stain was tested at 1:200 and gave excellent separation for organs tested; BM, blood and spleen.

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