Detection of EpCAM in Cancer Cells via Western Blot

Cancer bio
MDA
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-EpCAM antibody [E144]

Catalog Number:

ab32392

Our longstanding goal is to understand the role of biomarkers in different cell lines. Here we test the detection of EpCAM, an epithelial specific marker in different colon cancer cell lines using western blotting. From our analysis it is evident that the antibody specifically detects EpCAM expression and is very good.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human colon cancer cell line

Primary Incubation

1:1000 in 5% BSA

Blocking Agent

5% BSA

Secondary Incubation

1:1000 HRP

Tertiary Incubation

NA

Detection

Western blotting

Results Summary

Very good detection, bands as expected

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very clean antibody no background

The Bad

None observed

The Bottom Line

Outstanding antibody

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